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Protocols

The OHMR provides protocols to the research community for pet gut microbiome data generation. Below you will find documentation describing the contents of the stool sample collection kits tailored for collection from pets, collection instructions, and protocols for metagenomic and metabolomic data generation.

Sample collection and handling

  1.  Home pet stool collection* kit contents and protocol
  2.  Example clinical questionnaire* for metadata collection
  3.  Example owner’s questionnaire* for metadata collection

Molecular data generation

  1.  DNA extraction from stool**
  2.  16S rRNA gene amplicon sequencing protocol**
  3.  Metagenomics protocol**
  4.  Metabolomics protocol***
Provided by the *Harvard Chan Collection Core, **Hill’s Pet Nutrition, Inc., and the ***Broad Institute
 
Interested in conducting your own companion animal microbiome study? Learn more about the Harvard Chan Microbiome Collection Core and the Harvard Chan Microbiome Analysis Core.

One Health Microbiome Resource

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